megan o'brien

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We're in the process of filing for IP protection, so unfortunately, I can't dish the details (yet)!


Cell culture is an important molecular biology tool that allows in-vitro studies to be conducted prior to expensive and time consuming in-vivo experiments. Two-dimensional cell culture where cells are grown in a monolayer on a dish has been the standard technique since the inception of cell culture. However, this technique poorly mimics the in-vivo environment because of a lack of cell-cell interaction and the dynamic conditions that cells experience in-vivo, resulting in poor physiological predictions. It has been acknowledged that this does a poor job of replicating the anatomy and physiology of tissues found in the body. Three-dimensional cell cultures more closely replicate in-vivo conditions and have been investigated by several groups in recent years. Media perfusion systems have been developed to provide a dynamic system technique for cell growth. The coupling of perfusion and 3D cell culture results in a closer representation of the in-vivo environment, resulting in experiments being able to measure more realistic cell responses.


In collaboration with the Dana Farber Cancer Institute, we have developed a standardized perfusion bioreactor system that enables low-cost, easy-to-use, high throughput cell and tissue culture.

 
 
  • Final product
    Final product
  • Single fascicle prototype
    Single fascicle prototype
  • Full bioreactor model in Solidworks
    Full bioreactor model in Solidworks
  • Testing the final product
    Testing the final product
  • Final product
    Final product
Final product
Final product
  • Concept mockup and target vessels
    Concept mockup and target vessels